Producing selenomethionine-labeled proteins with a baculovirus expression vector system

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Expression of polyisoprenylated Ras proteins in the insect/baculovirus system.

Kus proteins and many related proteins possess the C-terminal motif C‘ys-AAX. where A is an aliphatic ;imino acid and X is itny iiniino acid. ‘I’his sequence directs ii prenyltransferiise. fh-nesyltransferase in the c;ise of ltas and geranylger;inyItransferase in most other ltas-related proteins. t o prenylate the cysteine residue 11-01. The nitture of the amino acid residue X appears t o deter...

متن کامل

Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette

Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus expression vector system (BEVS), including prolonged cell integrity after infection, improved protein integrity, and around 4-fold increase in r...

متن کامل

Construction of a Baculovirus-Silkworm Multigene Expression System and Its Application on Producing Virus-Like Particles

A new baculovirus-silkworm multigene expression system named Bombyx mori MultiBac is developed and described here, by which multiple expression cassettes can be introduced into the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome efficiently. The system consists of three donor vectors (pCTdual, pRADM and pUCDMIG) and an invasive diaminopimelate (DAP) auxotrophic recipient E. coli containin...

متن کامل

Protein Expression of the Bombyx mori Decapentaplegic Gene using the Baculovirus Expression Vector System

The Bombyx mori decapentaplegic gene is one of the conserved genes in vertebrate and invertebrates. The TGF-β superfamily contains conserved polypeptide growth factors that play important roles in different cellular processes such as proliferation, apoptosis, differentiation and cell-fate determination. The B. mori dpp gene shares genetic homology with hBMPs and Drosophila dpp. Until now, only ...

متن کامل

A novel baculovirus-derived promoter with high activity in the baculovirus expression system

The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in any other protein expression system, it is important to improve the production capabilities of this vector. The orf46 viral gene was iden...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Structure

سال: 1999

ISSN: 0969-2126

DOI: 10.1016/s0969-2126(00)80020-9